Chapter
A Novel CRISPR Approach to Analyze Genome Function
1:55:18 - 2:03:47 (08:29)
The podcast discusses a new CRISPR approach that cuts accessible regions of the genome enabling scientists to test all accessible regions for various functions. This new system has the ability to study research questions across a wide range of organisms, including humans, plants, and animals.
Clips
The development of microarray technology is allowing for the testing of thousands of genetic variants at a time, providing a fast and efficient way to measure gene expression and genotype.
1:55:18 - 1:57:34 (02:16)
Summary
The development of microarray technology is allowing for the testing of thousands of genetic variants at a time, providing a fast and efficient way to measure gene expression and genotype. This technology allows researchers to screen through millions of molecules and thousands of plates.
ChapterA Novel CRISPR Approach to Analyze Genome Function
Episode#133 – Manolis Kellis: Biology of Disease
PodcastLex Fridman Podcast
The epizomal assay allows testing of 10,000 different enhancers through a human cell culture and plasmids with the desired elements, by generating pieces with identical start and end barcodes.
1:57:34 - 2:00:16 (02:41)
Summary
The epizomal assay allows testing of 10,000 different enhancers through a human cell culture and plasmids with the desired elements, by generating pieces with identical start and end barcodes.
ChapterA Novel CRISPR Approach to Analyze Genome Function
Episode#133 – Manolis Kellis: Biology of Disease
PodcastLex Fridman Podcast
The use of massively parallel reporter assays (MPRAs) involves manipulating DNA sequences to understand their importance for gene expression.
2:00:16 - 2:03:47 (03:31)
Summary
The use of massively parallel reporter assays (MPRAs) involves manipulating DNA sequences to understand their importance for gene expression. MPRAs allowed the researchers to identify driver nucleotides and regulatory elements across seven million experiments at a time, enhancing gene annotation and speeding up the identification process.