Chapter
The Use of CRISPR in Gene Expression Research
1:38:07 - 1:45:19 (07:12)
Researchers have developed a way to use CRISPR-Cas9, a genome editing tool, to modify enhancers and see the impact on neighboring genes in a more controlled manner. This allows for a deeper understanding of gene expression and regulation.
Clips
An explanation of how the CRISPR targeting system and CRISPR-Cas9 protein allow for precise modification of DNA by either destroying or activating enhancers to see how it impacts gene expression.
1:38:07 - 1:42:42 (04:34)
Summary
An explanation of how the CRISPR targeting system and CRISPR-Cas9 protein allow for precise modification of DNA by either destroying or activating enhancers to see how it impacts gene expression. The process requires a team of biological engineers to design amino acid sequences that target specific sequences of the genome.
ChapterThe Use of CRISPR in Gene Expression Research
Episode#133 – Manolis Kellis: Biology of Disease
PodcastLex Fridman Podcast
The FTO gene has a large enhancer known as OB1 (Obesity One) which is a master regulator associated with progenitors of white, brown, and beige fat.
1:42:43 - 1:45:19 (02:36)
Summary
The FTO gene has a large enhancer known as OB1 (Obesity One) which is a master regulator associated with progenitors of white, brown, and beige fat. The IRX3 and IRX5 genes in the FTO locus are correlated positively with lipid metabolism and negatively with mitochondrial biogenesis.